Label-free multispectral monitoring of functional state: from embryos to human articular cartilage tissue (Conference Presentation)

Saabah B. Mahbub; Jared M. Campbell; Tiffany C. Y. Tan; Kylie R. Dunning; Abbas Habibalahi; Michael Bertoldo; Dale Goss; Lindsay Wu; Ewa M. Goldys

doi:10.1117/12.2545265

10 March 2020 Label-free multispectral monitoring of functional state: from embryos to human articular cartilage tissue (Conference Presentation)

Saabah B. Mahbub, Jared M. Campbell, Tiffany C. Y. Tan, Kylie R. Dunning, Abbas Habibalahi, Michael Bertoldo, Dale Goss, Lindsay Wu, Ewa M. Goldys

Author Affiliations +

Proceedings Volume 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020; 112510H (2020) https://doi.org/10.1117/12.2545265
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Automated, unbiased methods of non-invasive cell monitoring able to deal with complex biological heterogeneity are fundamentally important for biomedical research. Label-free imaging provides information about endogenous autofluorescent metabolites, enzymes and cofactors. Our multispectral fluorescence imaging technique allows precise quantification of native fluorophores in cells and tissues. This study uses label-free multispectral analysis to extract different fluorophores and redox ratio from single cells (oocytes, cultured cancer cells) as well as blastocyst embryos. Additionally, we characterise the molecular composition, structure and functional status of ex vivo healthy bovine and osteoarthritic human knee articular cartilage to assess 2 types of experimental treatments.

Conference Presentation

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Saabah B. Mahbub, Jared M. Campbell, Tiffany C. Y. Tan, Kylie R. Dunning, Abbas Habibalahi, Michael Bertoldo, Dale Goss, Lindsay Wu, and Ewa M. Goldys "Label-free multispectral monitoring of functional state: from embryos to human articular cartilage tissue (Conference Presentation)", Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112510H (10 March 2020); https://doi.org/10.1117/12.2545265

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