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10 March 2020 Molecular imaging of extracellular vesicles in vitro via Raman metabolic labelling (Conference Presentation)
Conor C. Horgan, Anika Nagelkerke, Thomas E. Whittaker, Valeria Nele, Lucia Massi, Ulrike Kauscher, Jelle Penders, Mads S. Bergholt, Steve R. Hood, Molly M. Stevens
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Proceedings Volume 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020; 112511G (2020) https://doi.org/10.1117/12.2541025
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Extracellular vesicles (EVs) are biologically derived nanovectors important for intercellular communication and trafficking, yet understanding of their underlying biological mechanisms remains poor. Advances have been hampered by both the complex biological origins of EVs and a lack of suitable imaging techniques. Here, we present a strategy for simultaneous in vitro imaging and molecular characterisation of EVs in 2D and 3D based on Raman spectroscopy and minimally-obstructive metabolic deuterium labelling. Metabolically-incorporated deuterium acts as a bio-orthogonal Raman-active tag for direct Raman identification of EVs and provides insights into their biocomposition and trafficking, with implications for their development as therapeutic delivery vectors.
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Conor C. Horgan, Anika Nagelkerke, Thomas E. Whittaker, Valeria Nele, Lucia Massi, Ulrike Kauscher, Jelle Penders, Mads S. Bergholt, Steve R. Hood, and Molly M. Stevens "Molecular imaging of extracellular vesicles in vitro via Raman metabolic labelling (Conference Presentation)", Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511G (10 March 2020); https://doi.org/10.1117/12.2541025
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KEYWORDS
Raman spectroscopy
In vitro testing
Molecular imaging
3D image processing
Molecular spectroscopy
Oxides
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