Virtual multi-focus optical-resolution photoacoustic microscope with extended depth of field using block DCT transform fusion

Xianlin Song; Jianshuang Wei; Xiaoquan Yang

doi:10.1117/12.2573294

15 June 2020 Virtual multi-focus optical-resolution photoacoustic microscope with extended depth of field using block DCT transform fusion

Xianlin Song, Jianshuang Wei, Xiaoquan Yang

Author Affiliations +

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 1152111 (2020) https://doi.org/10.1117/12.2573294
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

Photoacoustic microscopy with large depth of focus (DoF) is significant to the biomedical research. Here, we developed a virtual multi-focus optical-resolution photoacoustic microscope with extended depth of field by using block Discrete Cosine Transform (DCT) fusion. The source images from different focus is first 8 × 8 partitioned, and then DCT coefficients of each block can be calculated by using DCT transformation, and then the variance values of the corresponding blocks are calculated through DCT coefficients. The variance values are used as the activity level measures, blocks with large variances are selected. Finally, the fused image with virtual multi-focus is made up of blocks with the larger variances. Simulation and the in vivo imaging of zebra fish were performed to demonstrate that this method can extend the depth of field of PAM two times without the sacrifice of lateral resolution.

Citation Download Citation

Xianlin Song, Jianshuang Wei, and Xiaoquan Yang "Virtual multi-focus optical-resolution photoacoustic microscope with extended depth of field using block DCT transform fusion", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 1152111 (15 June 2020); https://doi.org/10.1117/12.2573294

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