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Autologous keratinocytes or stem cell based therapies are modern approaches for the treatment of skin loss in burn victims and chronic wound patients. The aim of this study is to identify depth-resolved structural changes in treated burn wounds using Spatial Frequency Domain Imaging (SFDI). When altering the investigated depth into tissue via the spatial frequency used in our calculations, we found changes in the scattering parameters for the treated samples. These scattering changes are correlated with histology, indicating a potential means to monitor re-epithelization and collagen formation during the treatment process across the entire wound area.
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Luigi Belcastro, Hanna Jonasson, Tomas Strömberg, Ahmed Elserafi, Rolf B. Saager, "Evaluation of cell therapy for burn wound using spatial frequency domain imaging," Proc. SPIE 11618, Photonics in Dermatology and Plastic Surgery 2021, 116180F (5 March 2021); https://doi.org/10.1117/12.2577192