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5 March 2021 Fluorescence dual-color fiberscope for monitoring neuron and astrocyte concurrent activities in freely-behaving animals
Rebecca W. Pak, Eric Hsu, Gian Molina-Castro, Dwight E. Bergles, Jin U. Kang
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Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 116291M (2021) https://doi.org/10.1117/12.2578947
Event: SPIE BiOS, 2021, Online Only
Abstract
Monitoring brain activity associated with natural physiological behavior is at the core of many neuroscience studies, yet many labs utilize imaging modalities that require anesthesia or immobilization of animals that cause unnatural stress and perturbed behavior. Instead, we separated the physiological system from the optical one using a fiber-optic tether, which allows the use of larger, optimized optical components while allowing mice to move freely thereby minimizing interference to their behavior. In vivo astrocyte activity, both spontaneous and stimulus-evoked was observed. The developed dual-color fluorescence system extended functionality to study multiple cell types concurrently.
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Rebecca W. Pak, Eric Hsu, Gian Molina-Castro, Dwight E. Bergles, and Jin U. Kang "Fluorescence dual-color fiberscope for monitoring neuron and astrocyte concurrent activities in freely-behaving animals", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116291M (5 March 2021); https://doi.org/10.1117/12.2578947
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KEYWORDS
Luminescence
Neurons
Head
Image processing
In vivo imaging
Microscopes
Neuroimaging
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