Single- and dual-objective light-sheet microscope architectures for cleared tissues: a simulation-based performance comparison

Kevin W. Bishop; Adam K. Glaser; Jonathan T. C. Liu

doi:10.1117/12.2577326

5 March 2021 Single- and dual-objective light-sheet microscope architectures for cleared tissues: a simulation-based performance comparison

Kevin W. Bishop, Adam K. Glaser, Jonathan T. C. Liu

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Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 116490R (2021) https://doi.org/10.1117/12.2577326
Event: SPIE BiOS, 2021, Online Only

Abstract

Light-sheet microscopy (LSM) is a powerful technique for rapid volumetric imaging of optically cleared specimens. Given the range of possible LSM configurations, designers would benefit from a systematic evaluation of the tradeoffs between different architectures. We present a simulation-based analysis of single- and dual-objective LSM designs for open-top, cleared-tissue imaging. System resolution and contrast are evaluated as functions of the crossing angle between the illumination and collection beams and each beam’s numerical aperture (NA). Our analysis reveals several key tradeoffs to guide designers in addition to potential advantages of a non-orthogonal dual-objective (NODO) architecture for moderate resolution imaging.

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Kevin W. Bishop, Adam K. Glaser, and Jonathan T. C. Liu "Single- and dual-objective light-sheet microscope architectures for cleared tissues: a simulation-based performance comparison", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 116490R (5 March 2021); https://doi.org/10.1117/12.2577326

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KEYWORDS

Computer architecture

Microscopes

Device simulation

Tissues

Biology

Microscopy

Objectives

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