Kilohertz full-frame two-photon imaging of cortical hemodynamics in vivo

Guanghan Meng; Jiang Lan Fan; Qinrong Zhang; Kevin K. Tsia; Na Ji

doi:10.1117/12.2578930

5 March 2021 Kilohertz full-frame two-photon imaging of cortical hemodynamics in vivo

Guanghan Meng, Jiang Lan Fan, Qinrong Zhang, Kevin K. Tsia, Na Ji

Proceedings Volume 11654, High-Speed Biomedical Imaging and Spectroscopy VI; 1165405 (2021) https://doi.org/10.1117/12.2578930
Event: SPIE BiOS, 2021, Online Only

Abstract

Uncovering the structure and function of cortical vascular network down to the level of capillaries can provide useful insights on brain physiology and pathology. Ideally, the probing method should allow concurrent observation of vascular morphology and hemodynamics, with sufficient spatiotemporal resolution to resolve individual capillaries and track blood cell motion in the scattering mammalian brain. By employing an all-optical scanning two-photon fluorescence microscope, we realized kilohertz full-frame recording of cortical blood vessels beyond 700 µm deep in the mouse brain and measured blood flow speeds up to 15 mm/s

Conference Presentation

Citation Download Citation

Guanghan Meng, Jiang Lan Fan, Qinrong Zhang, Kevin K. Tsia, and Na Ji "Kilohertz full-frame two-photon imaging of cortical hemodynamics in vivo", Proc. SPIE 11654, High-Speed Biomedical Imaging and Spectroscopy VI, 1165405 (5 March 2021); https://doi.org/10.1117/12.2578930

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