Multi-plane encoded light-sheet microscopy with acousto-optics

Alessandro Zunino; Francesco Garzella; Alberta Trianni; Peter Saggau; Paolo Bianchini; Alberto Diaspro; Martí Duocastella

doi:10.1117/12.2577559

5 March 2021 Multi-plane encoded light-sheet microscopy with acousto-optics

Alessandro Zunino, Francesco Garzella, Alberta Trianni, Peter Saggau, Paolo Bianchini, Alberto Diaspro, Martí Duocastella

Author Affiliations +

Proceedings Volume 11654, High-Speed Biomedical Imaging and Spectroscopy VI; 116540A (2021) https://doi.org/10.1117/12.2577559
Event: SPIE BiOS, 2021, Online Only

Abstract

Light-sheet microscopes with an extended depth of field (EDOF) offer a simple but powerful route toward fast volumetric imaging. However, methods for EDOF typically result in a loss of signal-to-noise ratio. Here, we propose a parallelization strategy as a simple solution. By illuminating multiple acoustically generated light sheets at different axial positions within the EDOF, and following an encoding sequence, information from several in-focus planes can be simultaneously retrieved. After applying a decoding algorithm, volumetric images are reconstructed with enhanced signal and level of detail. Our strategy paves the way for exploiting the full speed capabilities of EDOF light-sheet systems.

Conference Presentation

Citation Download Citation

Alessandro Zunino, Francesco Garzella, Alberta Trianni, Peter Saggau, Paolo Bianchini, Alberto Diaspro, and Martí Duocastella "Multi-plane encoded light-sheet microscopy with acousto-optics", Proc. SPIE 11654, High-Speed Biomedical Imaging and Spectroscopy VI, 116540A (5 March 2021); https://doi.org/10.1117/12.2577559

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