Extended depth of focus metal-induced energy transfer imaging with multiple axially shifted images

Wonsang Hwang; Dongeun Kim; Dugyoung Kim

doi:10.1117/12.2577339

5 March 2021 Extended depth of focus metal-induced energy transfer imaging with multiple axially shifted images

Wonsang Hwang, Dongeun Kim, Dugyoung Kim

Proceedings Volume 11658, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII; 1165808 (2021) https://doi.org/10.1117/12.2577339
Event: SPIE BiOS, 2021, Online Only

Abstract

Because the axial resolution of an optical microscope is in the order of the wavelength of light, imaging a nano-scale object is very challenging. Many nanometer-sectioning imaging technics such as total internal reflection fluorescence (TIRF) microscopy and metal-induced energy transfer (MIET) imaging have been invented to overcome this limitation. However, the measurement ranges of these methods along the axial direction are too short to cover even a single cell. Here we propose a new long-measurement-range nanometer-sectioning imaging scheme by using MIET, focal plane shifting, and sophisticated signal analysis. We have verified the principle and the feasibility of our proposed method by using an artificial sample; it is shown that the axial measurement range is extended from 100 nm to 500 nm.

Conference Presentation

Citation Download Citation

Wonsang Hwang, Dongeun Kim, and Dugyoung Kim "Extended depth of focus metal-induced energy transfer imaging with multiple axially shifted images", Proc. SPIE 11658, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII, 1165808 (5 March 2021); https://doi.org/10.1117/12.2577339

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