Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate

Vincent Maioli; Antoine Boniface; Pierre Mahou; Júlia Ferrer Ortas; Lamiae Abdeladim; Emmanuel Beaurepaire; Willy Supatto

doi:10.1117/12.2615701

7 December 2021 Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate

Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, Willy Supatto

Author Affiliations +

Proceedings Volume 11922, Advances in Microscopic Imaging III; 119220I (2021) https://doi.org/10.1117/12.2615701
Event: European Conferences on Biomedical Optics, 2021, Online Only

Abstract

We propose a framework to quantify photodamage in multiphoton light-sheet microscopy. Using cardiac imaging in live zebrafish embryos, we demonstrate an order of magnitude signal enhancement is safely obtained by adjusting the laser repetition rate.

Citation Download Citation

Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, and Willy Supatto "Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate", Proc. SPIE 11922, Advances in Microscopic Imaging III, 119220I (7 December 2021); https://doi.org/10.1117/12.2615701

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