A model for generating paired complete blood count and oblique back-illumination capillaroscopy data in tissue-realistic microfluidic chambers

Gregory N. McKay; Luojie Huang; Taylor L. Bobrow; Srivathsan Kalyan; Soojung Claire Hur; Sophie Lanzkron; Lydia H. Pecker; Alison R. Moliterno; Nicholas J. Durr

doi:10.1117/12.2609715

3 March 2022 A model for generating paired complete blood count and oblique back-illumination capillaroscopy data in tissue-realistic microfluidic chambers

Gregory N. McKay, Luojie Huang, Taylor L. Bobrow, Srivathsan Kalyan, Soojung Claire Hur, Sophie Lanzkron, Lydia H. Pecker, Alison R. Moliterno, Nicholas J. Durr

Author Affiliations +

Proceedings Volume 11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX; 119640G (2022) https://doi.org/10.1117/12.2609715
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Oblique back-illumination capillaroscopy (OBC) has recently demonstrated clear images of unlabeled human blood cells in vivo. Combined with deep learning-based algorithms, this technology may enable non-invasive blood cell counting and analysis as flowing red blood cells, platelets, and white blood cells can be observed in their native environment. To harness the full potential of OBC, new techniques and methods must be developed that provide ground truth data using human blood cells. Here we present such a model, where human blood cells with paired ground truth information are imaged flowing in a custom tissue-mimicking micro fluidic device. This model enables the acquisition of OBC datasets that will help with both training and validating machine learning models for applications including the complete blood count, specific blood cell classification, and the study of hematologic disorders such as anemia.

Conference Presentation

Citation Download Citation

Gregory N. McKay, Luojie Huang, Taylor L. Bobrow, Srivathsan Kalyan, Soojung Claire Hur, Sophie Lanzkron, Lydia H. Pecker, Alison R. Moliterno, and Nicholas J. Durr "A model for generating paired complete blood count and oblique back-illumination capillaroscopy data in tissue-realistic microfluidic chambers", Proc. SPIE 11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX, 119640G (3 March 2022); https://doi.org/10.1117/12.2609715

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