Monitoring glycation using the intrinsic fluorescence of biological fluorophores

Rhona Muir; Shareen Forbes; David J. S. Birch; Olaf J. Rolinski

doi:10.1117/12.2631649

3 October 2022 Monitoring glycation using the intrinsic fluorescence of biological fluorophores

Rhona Muir, Shareen Forbes, David J. S. Birch, Olaf J. Rolinski

Proceedings Volume 12201, UV and Higher Energy Photonics: From Materials to Applications 2022; 1220104 (2022) https://doi.org/10.1117/12.2631649
Event: SPIE Nanoscience + Engineering, 2022, San Diego, California, United States

Abstract

The high blood glucose levels associated with diabetes affect various cells and proteins in the body. In response to high blood glucose collagen and keratin proteins experience glycation. This work aims to establish if the intrinsic fluorescence of collagen and keratin could be used to monitor the glycation of said compounds, and thus offer an alternative method to monitoring long term glycaemic control. We have studied the evolution of the intrinsic fluorescence of both compounds in response to glucose in vitro, using steady state and time-resolved fluorescence spectroscopy techniques. Changes in the intrinsic fluorescence of both collagen and keratin were observed. For collagen, contrary to the traditional fluorescence intensity decay measurement at arbitrarily selected excitation and detection wavelengths, we conducted systematic wavelength- and time-resolved measurements to achieve time-resolved emission spectra (TRES). These showed changes in the intrinsic fluorescence kinetics, caused by both collagen aggregation and glycation. In keratin, the addition of glucose caused an increase in the fluorescence intensity at the characteristic wavelength of 460 nm, due to faster formation of new cross-links. The results also suggest that glucose may cause the formation of two new fluorescent complexes with peak fluorescence at ~525 nm and ~575 nm. In conclusion, monitoring the intrinsic fluorescence of collagen or keratin could be used as a method to monitor long term glycaemic control in patients with diabetes.

Citation Download Citation

Rhona Muir, Shareen Forbes, David J. S. Birch, and Olaf J. Rolinski "Monitoring glycation using the intrinsic fluorescence of biological fluorophores", Proc. SPIE 12201, UV and Higher Energy Photonics: From Materials to Applications 2022, 1220104 (3 October 2022); https://doi.org/10.1117/12.2631649

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Collagen

Glucose

Proteins

Skin

In vitro testing

Light scattering

Show All Keywords

Keywords/Phrases

Search In:

Publication Years