In-situ topographic characterization and mechanical mapping by divided-aperture confocal Brillouin microscopy

Yunhao Su; Weiqian Zhao; Lirong Qiu

doi:10.1117/12.2655746

9 January 2023 In-situ topographic characterization and mechanical mapping by divided-aperture confocal Brillouin microscopy

Yunhao Su, Weiqian Zhao, Lirong Qiu

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Proceedings Volume 12316, Advanced Optical Imaging Technologies V; 123160I (2023) https://doi.org/10.1117/12.2655746
Event: SPIE/COS Photonics Asia, 2022, Online Only

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Abstract

Brillouin scattering is an inelastic light scattering caused by local thermal vibration of materials. It can noninvasively detect the mechanical properties of materials such as stiffness, strain and elastic constant. Combined with confocal microscopy, confocal Brillouin microscope (CBM) can be used to detect the mechanical properties of micro-region materials with a non-contact manner. Therefore, it’s widely used in biomedical detection, material science and other frontier fields. However, due to the lack of fast and high-precision axial focusing ability of traditional CBM, the spatial resolution is limited, and the system stability is poor during long-time imaging process. Interference with specular reflected light can reduce the system's extinction ratio and sensitivity. These problems directly affect the accuracy of mechanical mapping results. A new type of divided-aperture confocal Brillouin microscope (DCBM) is proposed to improve its spatial resolution, stability and extinction ratio. The reflected light and scattered light are separated in space by divided-aperture, increased the extinction ratio by 20dB, and the reflected light is used to construct a confocal system to achieve the axial focusing accuracy of 5nm. The axial focusing ability of high sensitivity also significantly improves the spatial resolution and system stability. We used sheep myocardial tissue as a sample to verify the Brillouin mapping capability of the DCBM system. The fast in-situ imaging and high precision Brillouin mapping of the morphology and mechanical information of the sheep myocardial tissue was obtained at the same time. This technology provides a powerful tool for studying new phenomena in the fields of biology and materials.

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Yunhao Su, Weiqian Zhao, and Lirong Qiu "In-situ topographic characterization and mechanical mapping by divided-aperture confocal Brillouin microscopy", Proc. SPIE 12316, Advanced Optical Imaging Technologies V, 123160I (9 January 2023); https://doi.org/10.1117/12.2655746

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KEYWORDS

Confocal microscopy

Light scattering

Microscopy

Spatial resolution

Associative arrays

Imaging systems

Microscopes

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