A combination of photothermal and photodynamic therapies for treatment of prostate cancer

Yeongeun Kim; Hyun Wook Kang

doi:10.1117/12.2648551

14 March 2023 A combination of photothermal and photodynamic therapies for treatment of prostate cancer

Yeongeun Kim, Hyun Wook Kang

Proceedings Volume 12353, Advanced Photonics in Urology 2023; 1235305 (2023) https://doi.org/10.1117/12.2648551
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Among malignant tumors, prostate cancer is the second most found in men. Non-invasive methods using lasers, Photodynamic therapy (PDT) and Photothermal therapy (PTT), have been mainly studied as treatments. However, they have several limitations such as the intrinsic hypoxia of PDT and the unsafety for temperature of PTT. Therefore, in this study, PDT and PTT were combined by using conjugated photothermal agents (PA). For PDT-PTT, 635 nm laser light (1 W/cm² for 5 min) was used. DU145, human prostate cancer cell line was used for in in vitro tests. Cytotoxicity test using MTT and ROS detection test were conducted to evaluate the condition of PDT combined PTT. The cellular death was classified whether apoptosis or necrosis using flow cytometry with Annexin V/PI. PDT over a certain period confirmed to be less effective, and at the same time, the cell death rate increased by about 12 % when PDT and PTT were combined, proving the effect of tumoricidal effects compared to conventional PDT. During combined treatment, the cellular death of tumor was occurred through apoptosis and necrosis simultaneously. The current study demonstrated the PTT combined PDT increased anti-tumor effect with lower proliferation and inflammatory.

Conference Presentation

Citation Download Citation

Yeongeun Kim and Hyun Wook Kang "A combination of photothermal and photodynamic therapies for treatment of prostate cancer", Proc. SPIE 12353, Advanced Photonics in Urology 2023, 1235305 (14 March 2023); https://doi.org/10.1117/12.2648551

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