Application of multiphoton microscopy for studying liver fibrosis

Xiong Zhang; Xunbin Yu; Zheng Zhang; Jianxin Chen; Yannan Bai; Lianhuang Li

doi:10.1117/12.2687112

27 November 2023 Application of multiphoton microscopy for studying liver fibrosis

Xiong Zhang, Xunbin Yu, Zheng Zhang, Jianxin Chen, Yannan Bai, Lianhuang Li

Proceedings Volume 12770, Optics in Health Care and Biomedical Optics XIII; 127700V (2023) https://doi.org/10.1117/12.2687112
Event: SPIE/COS Photonics Asia, 2023, Beijing, China

Abstract

Liver fibrosis is a response to chronic liver damage, causing the accumulation of extracellular components like collagen fibers. Accurate evaluation of fibrosis is key for predicting disease prognosis. Multiphoton microscopy (MPM) is an advanced technique that allows label-free imaging of biomedical tissues using femtosecond laser-induced nonlinear optical effects. In this study, hepatic tissue samples were imaged via MPM and then imaging data were analyzed with Hover-Net, a convolutional neural network. We find that MPM has the ability to directly observe fibrotic changes, and also find that the number of portal bile ducts are positively related to liver fibrosis and can be automatically identified by the Hover-Net. These findings suggest MPM combining with deep learning can assess liver fibrosis quickly and reliably without the need for exogenous contrast agents.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Xiong Zhang, Xunbin Yu, Zheng Zhang, Jianxin Chen, Yannan Bai, and Lianhuang Li "Application of multiphoton microscopy for studying liver fibrosis", Proc. SPIE 12770, Optics in Health Care and Biomedical Optics XIII, 127700V (27 November 2023); https://doi.org/10.1117/12.2687112

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