Ex vivo detection of oral squamous cell carcinoma spread to lymph nodes using paired-agent fluorescence optical tomography: path to clinical translation

Anjalika Sharma; Cody C. Rounds; T. S. Nijboer; Floris Voskuil; Max J. H. Witjes; Jovan G Brankov; Kenneth M. Tichauer

doi:10.1117/12.3001099

12 March 2024 Ex vivo detection of oral squamous cell carcinoma spread to lymph nodes using paired-agent fluorescence optical tomography: path to clinical translation

Anjalika Sharma, Cody C. Rounds, T. S. Nijboer, Floris Voskuil, Max J. H. Witjes, Jovan G Brankov, Kenneth M. Tichauer

Author Affiliations +

Proceedings Volume 12825, Molecular-Guided Surgery: Molecules, Devices, and Applications X; 1282507 (2024) https://doi.org/10.1117/12.3001099
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

In the United States, the annual incidence of oral squamous cell carcinoma (OSCC) exceeds 50,000 cases. Primary tumor resection remains the first line of treatment in these patients yet follow up neck dissection and chemoradiation treatment may be indicated if cancer has spread to tumor draining lymph nodes. There is a push to minimize morbidity from neck dissection by sentinel lymph node biopsy, where only the first lymph node(s) draining the primary tumor are excised and evaluated for cancer spread. However, with current pathology methods, results are not available to surgeons until patients have been sent home. In response, we are developing a method to rapidly stain and image whole excised lymph nodes in less than 30 min, so surgeons can react to positive cases while patients are still on the operating table. Here we present a human head and neck cancer spheroid model implanted in porcine lymph nodes as a means evaluating the potential for our staining and imaging protocols to rapidly identify cancer burden in lymph nodes.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Anjalika Sharma, Cody C. Rounds, T. S. Nijboer, Floris Voskuil, Max J. H. Witjes, Jovan G Brankov, and Kenneth M. Tichauer "Ex vivo detection of oral squamous cell carcinoma spread to lymph nodes using paired-agent fluorescence optical tomography: path to clinical translation", Proc. SPIE 12825, Molecular-Guided Surgery: Molecules, Devices, and Applications X, 1282507 (12 March 2024); https://doi.org/10.1117/12.3001099

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