Quantitative OCT angiography for dynamic volumetric analysis in embryonic cardiovascular system

Michaela A. McCown; Irina V. Larina

doi:10.1117/12.3005714

12 March 2024 Quantitative OCT angiography for dynamic volumetric analysis in embryonic cardiovascular system

Michaela A. McCown, Irina V. Larina

Proceedings Volume 12841, Dynamics and Fluctuations in Biomedical Photonics XXI; 1284103 (2024) https://doi.org/10.1117/12.3005714
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Congenital heart defects represent one in three congenital defects and are present in an estimated 1.8% of newborns worldwide. Mouse models provide an irreplaceable resource for studying mammalian development and early cardiogenesis. Early dynamics of circulation during heart development are understood to influence heart formation, but quantitative assessment of spatially and temporally resolved blood flow in the highly dynamic embryonic hearts remains challenging. Optical coherence tomography (OCT) uniquely provides the high speed, spatial resolution, and imaging depth necessary to study biomechanics early in heart development. Building off advancements in Doppler OCT and quantitative OCT angiography, we present dynamic, volumetric (4D) speed analysis of blood flow in the embryonic cardiovascular system. Our new flow tracking method is based on time-at-pixel measurements, blood cell size statistics, and the periodicity of the cardiac cycle. We characterize the effects of detection thresholds to account for variation in signal intensity, such as due to lower light penetration over tissue depth throughout the heart or when comparing between embryos. We incorporate segmentation methods using speckle variance between cycles to expand the analysis from manually defined blood vessels to dynamic regions of blood flow. With these advancements, we quantify blood flow speed within the embryonic mouse heart as it beats. The presented method will allow biomechanical studies of early blood flow in regulating mammalian heart development.

Conference Presentation

Citation Download Citation

Michaela A. McCown and Irina V. Larina "Quantitative OCT angiography for dynamic volumetric analysis in embryonic cardiovascular system", Proc. SPIE 12841, Dynamics and Fluctuations in Biomedical Photonics XXI, 1284103 (12 March 2024); https://doi.org/10.1117/12.3005714

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