Fluorescence detection of tumors: studies on the early diagnosis of microscopic lesions in preclinical and clinical studies

Thomas S. Mang; Carolyn McGinnis; David H. Crean; S. Khan; Charles Liebow

doi:10.1117/12.44050

1 June 1991 Fluorescence detection of tumors: studies on the early diagnosis of microscopic lesions in preclinical and clinical studies

Thomas S. Mang, Carolyn McGinnis, David H. Crean, S. Khan, Charles Liebow

Author Affiliations +

Proceedings Volume 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques; (1991) https://doi.org/10.1117/12.44050
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

The growth of microscopic tumor lesions at or beyond treatment field lesions poses major problems in the diagnosis and curative treatment of numerous cancers. Early detection techniques which clearly define the extent of condemned or field spread of disease may improve the primary treatment of the disease. In vivo fluorescence photometry is a non-imaging technique which digitally displays relative fluorescence values in volts. The sensitivity of the instrument has allowed the detection of micrometastases in both pre-clinical and clinical studies using drug doses that are 80-90 lower than those used therapeutically. This technique is now being applied in preliminary experiments to the hamster cheek pouch models to (1) discern varying grades of dysplasia; (2) levels of uptake of the drug in normal growing and quiescent tumors. Results will be shown in two models in which this technique has shown to be efficacious preclinically in the Pollard rat adenocarcinoma model in which micrometastases in the lymph node have been detected, and preliminary studies involving the hamster cheek pouch model in which the pouch is painted with 9, 10 dimethyl-1, 2-benzanthracene (DMBA) for initiation and promotion of tumors. Clinically results will be shown in which fluorescence detection, confirmed by biopsy and histopathological examination, was capable of detecting the existence of micrometastatic involvement of less than 100 cells.

Citation Download Citation

Thomas S. Mang, Carolyn McGinnis, David H. Crean, S. Khan, and Charles Liebow "Fluorescence detection of tumors: studies on the early diagnosis of microscopic lesions in preclinical and clinical studies", Proc. SPIE 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques, (1 June 1991); https://doi.org/10.1117/12.44050

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