Confocal differential phase contrast adaptor for beam scanning confocal microscope

Victor Chen; Edward Hurley

doi:10.1117/12.59577

26 June 1992 Confocal differential-phase-contrast adaptor for beam-scanning confocal microscope

Victor Chen, Edward Hurley

Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59577
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States

Abstract

We demonstrate the implementation of the confocal differential phase contrast imaging technique to a commercial beam scanning confocal microscope. Using this imaging mode on a scanning stage confocal microscope the complex texture of reflectance images seem very much simplified and `improved.' However, as a microscope user rather than a microscope developer, the accessible confocal microscopes usually employ beam scanning. We felt it important to implement this contrast mode to the beam scanning instrument so that useful reflectance mode confocal images could more easily be obtained. We implemented the optical scheme described by Wilson. Cogswell and Sheppard further show on a scanning beam confocal microscope the power of the differential phase contrast mode for the study of biological specimens by implementing `unsharp' masking to selectively enhance image detail in difficult specimens. We are able to do this type of enhancement to a limited extent using our implementation of the confocal differential phase contrast technique to a commercial beam scanning confocal microscope.

Citation Download Citation

Victor Chen and Edward Hurley "Confocal differential-phase-contrast adaptor for beam-scanning confocal microscope", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59577

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