Determination of optical constants and morphological parameters of biological particles from multiple light-scattering characteristics

Alphiya Ya. Khairullina

doi:10.1117/12.148354

21 July 1993 Determination of optical constants and morphological parameters of biological particles from multiple light-scattering characteristics

Alphiya Ya. Khairullina

Author Affiliations +

Proceedings Volume 1884, Static and Dynamic Light Scattering in Medicine and Biology; (1993) https://doi.org/10.1117/12.148354
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Unified approach is suggested to determine the real (n) and imaginary (x) parts of refractive index and average size r of biological particle suspensions, biopolymers, and so on for a wide range of sizes (r equals 10^-1 - 10² micrometers , n equals 1.02 - 1.15 and x equals 10 ^-6 - 10^-4). The values to be measured are the diffuse reflection and transmission coefficients and the temporal spectra scattering intensity fluctuations of optically thick layers in the case of weak absorption (this condition is determined experimentally). The sensitivity of the proposed approach for the determination of n and x (as well as the average cosine of the phase function of light scattering <cos(phi) > and radiation pressure cross section (beta) for finding the force acting on the particle) is very high and exceeds the known methods. The methods of determining n, x, r, <cos(phi) >, (beta) have been evaluated for suspensions of erythrocytes, blood substitute (emulsions of PFC), and latexes.

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Alphiya Ya. Khairullina "Determination of optical constants and morphological parameters of biological particles from multiple light-scattering characteristics", Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993); https://doi.org/10.1117/12.148354

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