Fluorescence-lifetime-based sensing: applications to clinical chemistry and cellular imaging

Joseph R. Lakowicz; Henryk Szmacinski; Richard B. Thompson

doi:10.1117/12.146718

24 June 1993 Fluorescence-lifetime-based sensing: applications to clinical chemistry and cellular imaging

Joseph R. Lakowicz, Henryk Szmacinski, Richard B. Thompson

Proceedings Volume 1895, Ultrasensitive Laboratory Diagnostics; (1993) https://doi.org/10.1117/12.146718
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Measurements of fluorescence lifetimes, rather than intensity or intensity ratios, offer many advantages in clinical chemistry and imaging. However, measurements of time-resolved fluorescence are normally associated with complex laser light sources and instrumentation. In this lecture, we show how emerging technology is enabling the design and use of simple instrumentation for time-resolved fluorescence. In particular, it is now possible to imagine lifetime-based measurements of blood gases and blood glucose, and lifetime imaging of calcium and other ions in microscopic samples.

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Joseph R. Lakowicz, Henryk Szmacinski, and Richard B. Thompson "Fluorescence-lifetime-based sensing: applications to clinical chemistry and cellular imaging", Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); https://doi.org/10.1117/12.146718

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