Investigations in high-speed DNA sequencing

Rainer Erdmann; Claus A. M. Seidel; F. Schubert; Edgar O. Klose

doi:10.1117/12.146726

24 June 1993 Investigations in high-speed DNA sequencing

Rainer Erdmann, Claus A. M. Seidel, F. Schubert, Edgar O. Klose

Proceedings Volume 1895, Ultrasensitive Laboratory Diagnostics; (1993) https://doi.org/10.1117/12.146726
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The application of a rapid time correlated single photon counting (TCSPC) apparatus on the investigation of fluorescent labelled DNA is presented. It could be shown that the investigated dye coumarin 120 can be used as a marker for a one dye-one lane electrophoresis-concept in DNA sequencing. This is possible because of the interaction between the dye and the four different DNA bases, which is based on a photoinduced electron transfer. A distinguishing of the bases is possible because of the different decay times. A fluorescein derivative also showed excellent fluorescence after labelling. It can be used as a marker for common electrophoresis- concepts, but the interaction was too weak for a secure base distinguishing a one dye-one lane. Now further investigations are under way to find new labeling positions resulting in stronger interactions. Additionally, we are looking for dyes in the red spectral range to improve the sensitivity of detection.

Citation Download Citation

Rainer Erdmann, Claus A. M. Seidel, F. Schubert, and Edgar O. Klose "Investigations in high-speed DNA sequencing", Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); https://doi.org/10.1117/12.146726

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