Determination of the kinetic response and absolute sensitivity of a fiber-optic immunoassay

Sandra F. Feldman; E. E. Uzgiris

doi:10.1117/12.170659

10 March 1994 Determination of the kinetic response and absolute sensitivity of a fiber-optic immunoassay

Sandra F. Feldman, E. E. Uzgiris

Proceedings Volume 2068, Chemical, Biochemical, and Environmental Fiber Sensors V; (1994) https://doi.org/10.1117/12.170659
Event: Optical Tools for Manufacturing and Advanced Automation, 1993, Boston, MA, United States

Abstract

We have used an evanescently excited fiber optic immunoassay to study the kinetic response of an immunosensor and determine its absolute sensitivity. We have estimated that when binding sites are saturated approximately 10¹¹ antigen are bound to a fiber probe of 0.57 cm² active area using optical techniques and without resorting to a radioassay. The number of labeled antigen for a threshold signal is approximately 10⁹. We can easily detect fluorescently labeled antigen at subnanomolar concentrations and have measured the probe response to antigens over more than two orders of magnitude. The overall response time for this system and labeled antigen (M_w approximately equals 100,000) is on the order of 10 minutes for a 40 pM solution. At higher concentrations, a response may be obtained in a few minutes.

Citation Download Citation

Sandra F. Feldman and E. E. Uzgiris "Determination of the kinetic response and absolute sensitivity of a fiber-optic immunoassay", Proc. SPIE 2068, Chemical, Biochemical, and Environmental Fiber Sensors V, (10 March 1994); https://doi.org/10.1117/12.170659

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