Correction of blood perfusion influences on NADH fluorescence spectroscopic measurements

Wolfgang Hoehne; Werner Schramm

doi:10.1117/12.166826

15 January 1994 Correction of blood perfusion influences on NADH fluorescence spectroscopic measurements

Wolfgang Hoehne, Werner Schramm

Proceedings Volume 2081, Optical Biopsy; (1994) https://doi.org/10.1117/12.166826
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

In vivo measurement of NADH autofluorescence is strongly disturbed by changes in the Hb absorption of excitation and fluorescence light. An approach to this problem is based on the diffuse reflectance measurement of 805 nm (isobestic point of Hb/HbO₂). According a Kubelka-Munk calculation an increase in Hb/HbO₂ concentration leads to a decrease of diffuse reflectance. This gives correction terms for the measured autofluorescence data. An equipment to perform such measurements will be presented.

Citation Download Citation

Wolfgang Hoehne and Werner Schramm "Correction of blood perfusion influences on NADH fluorescence spectroscopic measurements", Proc. SPIE 2081, Optical Biopsy, (15 January 1994); https://doi.org/10.1117/12.166826

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