Methods and application of time-resolved fluorescence microscopy

Harold K. Seidlitz; Jurina M. Wessels

doi:10.1117/12.167426

1 February 1994 Methods and application of time-resolved fluorescence microscopy

Harold K. Seidlitz, Jurina M. Wessels

Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167426
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

The combination of fluorescence microscopy and time-resolved techniques opens up new possibilities in studying pharmacokinetics and localization of various drugs on a cellular scale. The fluorescence decay time of many molecules is modulated by the physical and chemical properties of the microenvironment, such as viscosity, polarity, pH-value, etc. The alternation of the decay time can also be used to pursue chemical transformations of a drug like degradation, monomerization, or protonation. This paper presents applications of this method from the field of photosensitizing dyes used in photodynamic therapy.

Citation Download Citation

Harold K. Seidlitz and Jurina M. Wessels "Methods and application of time-resolved fluorescence microscopy", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); https://doi.org/10.1117/12.167426

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