Optical trapping and manipulation of single cells and motile microorganisms by laser diode radiation

Carlo Frediani; Cesare Ascoli; S. Lucia; P. Verkerk; L. Guidoni; A. Fioretti; Ennio Arimondo

doi:10.1117/12.197506

22 December 1994 Optical trapping and manipulation of single cells and motile microorganisms by laser diode radiation

Carlo Frediani, Cesare Ascoli, S. Lucia, P. Verkerk, L. Guidoni, A. Fioretti, Ennio Arimondo

Proceedings Volume 2328, Biomedical Optoelectronic Devices and Systems II; (1994) https://doi.org/10.1117/12.197506
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Electromagnetic fields, emitted by laser sources, have been utilized in recent years for controlling the position and velocity of atoms, ions and microscopic neutral particles. In 1987 Ashkin has shown for the first time that cells too can be trapped by using a focused beam of laser radiation. The trapping is due to the interation between the electric dipole induced by the laser electric field in the cell and the electric field itself. In order to maximize the trapping effect and to avoid damage to the cells caused by excessive heating, the laser wavelength must be far from the absorption bands for both the cells and the solution where cells are kept, usually water. Our preliminary experiments, utilizing a 100 mW laser diode at 850 nm with suitable focusing, show that also free swimming (up to 100 micrometers /s) protozoa (Dunaliella salina) can be easily trapped, without apparent damage. The experimental set-up and the experiments on motile micro-organisms are presented. Possible biomedical applications are discussed.

Citation Download Citation

Carlo Frediani, Cesare Ascoli, S. Lucia, P. Verkerk, L. Guidoni, A. Fioretti, and Ennio Arimondo "Optical trapping and manipulation of single cells and motile microorganisms by laser diode radiation", Proc. SPIE 2328, Biomedical Optoelectronic Devices and Systems II, (22 December 1994); https://doi.org/10.1117/12.197506

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KEYWORDS

Optical tweezers

Semiconductor lasers

Absorption

Microorganisms

Objectives

Particles

Laser beam propagation

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