Peculiarities of backscattering nephelometry of whole blood applied to clinical diagnostics

Olga M. Ryaboshapka; Nikolai N. Firsov; Alexander V. Priezzhev

doi:10.1117/12.200874

1 February 1995 Peculiarities of backscattering nephelometry of whole blood applied to clinical diagnostics

Olga M. Ryaboshapka, Nikolai N. Firsov, Alexander V. Priezzhev

Proceedings Volume 2329, Optical and Imaging Techniques in Biomedicine; (1995) https://doi.org/10.1117/12.200874
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

The aggregational properties of erythrocytes in whole blood are studied by backscattering nephelometry during their spontaneous aggregation and disaggregation in vitro. As the process of aggregate formation develops from pair via linear to 3-D aggregates the parameters of the aggregation kinetics are uniquely related to the aggregate morphology although the mechanisms of aggregation under pathologies are not quite clear. Different manifestations of disaggregation processes registered by the backscattering technique show that the same level of light scattering can correspond to different morphological states of aggregates and their behavior in shear flow. The variety of disaggregation kinetics obtained from different samples reflects different features of alteration of aggregational properties of erythrocytes and hydrodynamic behavior of aggregates under shear stress in the Couette flow. In case of some pathologies we observe different disaggregation effects such as the increase of aggregation at small shear rates (2.5 sec^-1 in our set-up), the transitional processes of disaggregation at stepwise increase of shear rate, aggregate destruction with possible increase of durability of 3-D aggregates or that of small aggregates, the hysteresis loops at direct and reverse change of shear rate. These effects are sensitive to the volume concentration of erythrocytes.

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Olga M. Ryaboshapka, Nikolai N. Firsov, and Alexander V. Priezzhev "Peculiarities of backscattering nephelometry of whole blood applied to clinical diagnostics", Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); https://doi.org/10.1117/12.200874

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KEYWORDS

Blood

Backscatter

Pathology

Diagnostics

In vitro testing

Scattering

Light scattering

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