From in vitro to in vivo by dynamic multiwavelength imaging

Daniel L. Farkas; Byron T. Ballou; Gregory W. Fisher; D. Lansing Taylor

doi:10.1117/12.206015

3 April 1995 From in vitro to in vivo by dynamic multiwavelength imaging

Daniel L. Farkas, Byron T. Ballou, Gregory W. Fisher, D. Lansing Taylor

Proceedings Volume 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics; (1995) https://doi.org/10.1117/12.206015
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

There is a clear trend today towards non-invasive, dynamic, digital approaches to biomedical imaging, and a need for even higher resolution. Light is particularly well suited for such investigations, as its temporal, spatial and intensity range are unparalleled. A convergence of new capabilities from fields as diverse as electronics, optics, molecular biology, computer science and dye chemistry have transformed light microscopy from a traditional, static, 2D tool into a highly useful, dynamic, 3D research capability for biology and medicine. We believe that the understanding of certain fundamental biological functions by dynamic mapping of events in living systems is within reach, based on novel, interdisciplinary methods. For imaging molecular events with high resolution (live cells, in vitro), light microscopy has continued to improve in performance, and we survey here some of our recent progress. The same dynamic mapping can be extended to organs, whole animals and humans, by monitoring molecules labeled with the long-wavelength dyes that proved useful in microscopy. We report here results obtained by in vivo imaging of fluorescently labeled monoclonal antibodies, indicative of tumor location and evolution in nude mice.

Citation Download Citation

Daniel L. Farkas, Byron T. Ballou, Gregory W. Fisher, and D. Lansing Taylor "From in vitro to in vivo by dynamic multiwavelength imaging", Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); https://doi.org/10.1117/12.206015

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available