Laser-induced fluorescence detection of dysplasia in Barrett's esophagus

Masoud Panjehpour; Bergein F. Overholt M.D.; Tuan Vo-Dinh; Donna H. Edwards; Paul F. Buckley III; Joseph F. DeCosta; Rodger C. Haggitt M.D.

doi:10.1117/12.237584

5 April 1996 Laser-induced fluorescence detection of dysplasia in Barrett's esophagus

Masoud Panjehpour, Bergein F. Overholt M.D., Tuan Vo-Dinh, Donna H. Edwards, Paul F. Buckley III, Joseph F. DeCosta, Rodger C. Haggitt M.D.

Author Affiliations +

Proceedings Volume 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy; (1996) https://doi.org/10.1117/12.237584
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

A study was conducted to determine whether laser-induced fluorescence could detect high grade dysplasia in Barrett's esophagus. Four-hundred-ten nm laser light was used to induce autofluorescence of Barrett's mucosa in 36 patients during routine endoscopy. The spectra were analyzed using the Differential Normalized Fluorescence (DNF) Index technique to differentiate high grade dysplasia from either low grade or non-dysplastic mucosa. Each spectrum was classified as either premalignant or benign using two different DNF indices. Analyzing the fluorescence spectra from all patients using one DNF Index, 96% of non- dysplastic Barrett's samples classified as benign tissue. All low grade dysplasia samples classified as benign. Ninety percent of high grade dysplasia samples classified as premalignant. Twenty-eight percent of mixed low grade/focal high grade dysplasia samples classified as premalignant. In summary, high grade dysplasia in Barrett's esophagus patients can be detected by endoscopic laser-induced fluorescence spectroscopy using differential normalized fluorescence technique.

Citation Download Citation

Masoud Panjehpour, Bergein F. Overholt M.D., Tuan Vo-Dinh, Donna H. Edwards, Paul F. Buckley III, Joseph F. DeCosta, and Rodger C. Haggitt M.D. "Laser-induced fluorescence detection of dysplasia in Barrett's esophagus", Proc. SPIE 2679, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, (5 April 1996); https://doi.org/10.1117/12.237584

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