Cell damage in two-photon microscopes

Karsten Koenig; Peter T. C. So; William W. Mantulin; Enrico Gratton

doi:10.1117/12.260794

6 December 1996 Cell damage in two-photon microscopes

Karsten Koenig, Peter T. C. So, William W. Mantulin, Enrico Gratton

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260794
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

We report on power thresholds for cell damage in femtosecond two-photon NIR microscopes and discuss interactions between vital cells and high intense femtosecond kW pulses in the TW/cm² (10¹² W/cm²) range. The influence of femtosecond NIR pulses on cell metabolism and cell vitality was studied by autofluorescence imaging, morphology studies, employment of vitality kits and sensitive cell cloning assays. We show that cells remain unaffected by high intense NIR femtosecond pulses below certain power thresholds. This allows nondestructive nonlinear 3D fluorescence imaging of vital cells. Above these thresholds, giant cell growth, failed cell division, complete cell destruction, and intracellular plasma formation occur.

Citation Download Citation

Karsten Koenig, Peter T. C. So, William W. Mantulin, and Enrico Gratton "Cell damage in two-photon microscopes", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260794

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