Analysis of drugs in human tissues by supercritical fluid extraction/immunoassay

Kenneth G. Furton; Alberta Sabucedo; Joseph Rein; W. Lee Hearn

doi:10.1117/12.266308

10 February 1997 Analysis of drugs in human tissues by supercritical fluid extraction/immunoassay

Kenneth G. Furton, Alberta Sabucedo, Joseph Rein, W. Lee Hearn

Proceedings Volume 2941, Forensic Evidence Analysis and Crime Scene Investigation; (1997) https://doi.org/10.1117/12.266308
Event: Enabling Technologies for Law Enforcement and Security, 1996, Boston, MA, United States

Abstract

A rapid, readily automated method has been developed for the quantitative analysis of phenobarbital from human liver tissues based on supercritical carbon dioxide extraction followed by fluorescence enzyme immunoassay. The method developed significantly reduces sample handling and utilizes the entire liver homogenate. The current method yields comparable recoveries and precision and does not require the use of an internal standard, although traditional GC/MS confirmation can still be performed on sample extracts. Additionally, the proposed method uses non-toxic, inexpensive carbon dioxide, thus eliminating the use of halogenated organic solvents.

Citation Download Citation

Kenneth G. Furton, Alberta Sabucedo, Joseph Rein, and W. Lee Hearn "Analysis of drugs in human tissues by supercritical fluid extraction/immunoassay", Proc. SPIE 2941, Forensic Evidence Analysis and Crime Scene Investigation, (10 February 1997); https://doi.org/10.1117/12.266308

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