Gallstone identification by fluorescence spectroscopy

Asima Pradhan; B. V. Laxmi; Sidhartha S. Jena; P. K. Khulbe; Hari Datt Bist; Asha Agarwal

doi:10.1117/12.305367

16 April 1998 Gallstone identification by fluorescence spectroscopy

Asima Pradhan, B. V. Laxmi, Sidhartha S. Jena, P. K. Khulbe, Hari Datt Bist, Asha Agarwal

Proceedings Volume 3250, Optical Biopsy II; (1998) https://doi.org/10.1117/12.305367
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Gallstones have been classified as being cholesterol type and pigment type. The classification is important for diet control of the patient to avoid recurrence of the stone. Spectroscopy is a sensitive technique to determine the composition of the gallstone both in-vitro and in-vivo. this work deals with the fluorescence spectroscopy of gallstone. For fluorescence spectroscopic studies of gallstone, samples were excited with 5 mw of 488 nm line of argon-ion laser and spectra were recorded with a SPEX 1877E triplemate attached with a cooled PMT and DM3000R data acquisition system. Fluorescence spectra from pure cholesterol and bilirubin were also recorded for comparison. Different types of gallstones: mixed, cholesterol, pigment type were studied. All spectra exhibited a very broad band, 500 to 800 nm and sometimes two bands, depending on type of stone. Pure cholesterol shows three prominent fluorescence peaks at 513, 550, 583 nm along with two peaks at approximately 568 and 586 nm. Pure bilirubin shows prominent peak at 628 nm, without any Raman line. From fluorescence spectra different types of stones are identified. Different gallstones studied show a mixture of cholesterol and bilirubin types and the ratio of the two varies from one sample type to another.

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Asima Pradhan, B. V. Laxmi, Sidhartha S. Jena, P. K. Khulbe, Hari Datt Bist, and Asha Agarwal "Gallstone identification by fluorescence spectroscopy", Proc. SPIE 3250, Optical Biopsy II, (16 April 1998); https://doi.org/10.1117/12.305367

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KEYWORDS

Biliary calculi

Luminescence

Fluorescence spectroscopy

Raman spectroscopy

Spectroscopy

Data acquisition

In vitro testing

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