Second harmonic and two-photon fluorescence histology of tissues

Yici Guo; Feng Liu; Quan-Zhen Wang; Niclolay N. Zhadin; Peng Pei Ho; Howard E. Savage; D. Harris; Peter G. Sacks; Stimson P. Schantz M.D.; Robert R. Alfano

doi:10.1117/12.305381

16 April 1998 Second harmonic and two-photon fluorescence histology of tissues

Yici Guo, Feng Liu, Quan-Zhen Wang, Niclolay N. Zhadin, Peng Pei Ho, Howard E. Savage, D. Harris, Peter G. Sacks, Stimson P. Schantz M.D., Robert R. Alfano

Author Affiliations +

Proceedings Volume 3250, Optical Biopsy II; (1998) https://doi.org/10.1117/12.305381
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Second harmonic and two photon fluorescence generated by focused 10 fs ultrashort laser pulses were used to image subsurface structures of highly scattering biological tissues. By scanning the focal point of the incident laser beam, 2D sectional images of the tissues subsurface structure properties, such as symmetry and molecular compositions are obtained.

Citation Download Citation

Yici Guo, Feng Liu, Quan-Zhen Wang, Niclolay N. Zhadin, Peng Pei Ho, Howard E. Savage, D. Harris, Peter G. Sacks, Stimson P. Schantz M.D., and Robert R. Alfano "Second harmonic and two-photon fluorescence histology of tissues", Proc. SPIE 3250, Optical Biopsy II, (16 April 1998); https://doi.org/10.1117/12.305381

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available