Time-resolved phosphorescence anisotropy and photobleaching recovery characterization of protein aggregation induced by paraformaldehyde fixation of cells

B. George Barisas; William F. Wade; Deborah A. Roess

doi:10.1117/12.307070

1 May 1998 Time-resolved phosphorescence anisotropy and photobleaching recovery characterization of protein aggregation induced by paraformaldehyde fixation of cells

B. George Barisas, William F. Wade, Deborah A. Roess

Author Affiliations +

Proceedings Volume 3256, Advances in Optical Biophysics; (1998) https://doi.org/10.1117/12.307070
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Antigen presentation by MHC class II molecules can be enhanced by paraformaldehyde fixation of antigen-presenting cells prior to assay. This treatment might be expected to aggregate membrane proteins and thus stabilize and strengthen transient protein-protein interactions involved in intercellular cooperation. Rotational and lateral dynamics of the MHC class II antigen I-A^d on A20 cells fixed with various concentrations of paraformaldehyde were examined by time- resolved phosphorescence anisotropy and fluorescence photobleaching recovery, respectively. Probes were erythrosin and tetramethylrhodamine conjugates of MKD6 Fab fragments. Increasing concentrations of paraformaldehyde progressively increased I-A^d's limiting anisotropy at 4 degrees Celsius above the value of 0.042 seen in untreated cells while leaving the rotational correlation time of 22 microsecond unchanged. On the other hand, translational diffusion coefficients decreased from about 2 X 10^-10 cm² sec^-1 while recovery remained unchanged at 40 - 50%. Together these results suggest that fixation crosslinks class II molecules with each other or with other membrane proteins into structures large enough (greater than 500,000 kDa) to appear rotationally immobile but small enough to diffuse translationally with size-dependent rates. Fixation effects on both class II rotation and lateral diffusion are half-maximal at paraformaldehyde concentrations of approximately 0.2%. Possible relations between biology of class II effector functions and physical sizes of fixation-induced aggregates are discussed.

Citation Download Citation

B. George Barisas, William F. Wade, and Deborah A. Roess "Time-resolved phosphorescence anisotropy and photobleaching recovery characterization of protein aggregation induced by paraformaldehyde fixation of cells", Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); https://doi.org/10.1117/12.307070

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Molecules

FDA class II medical device development

Diffusion

Proteins

Anisotropy

Phosphorescence

Luminescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years