Imaging of human colon carcinoma thin sections by FT-IR microspectrometry

Peter Lasch; Wolfgang Waesche; W. J. McCarthy; Gerhard J. Mueller; Dieter Naumann

doi:10.1117/12.306083

24 April 1998 Imaging of human colon carcinoma thin sections by FT-IR microspectrometry

Peter Lasch, Wolfgang Waesche, W. J. McCarthy, Gerhard J. Mueller, Dieter Naumann

Proceedings Volume 3257, Infrared Spectroscopy: New Tool in Medicine; (1998) https://doi.org/10.1117/12.306083
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

FTIR microspectroscopic maps of unstained colon carcinoma thin sections were obtained on a conventional IR microscope equipped with an automatic x, y stage, or alternatively by using a MCT focal plane array detector system. IR data were analyzed by different image re-assembling techniques. One main goal of the present study was to test the influence of different spectra data compression approaches on the quality of the FTIR images. The images, re-assembled by Principal component analysis (PCA) on the basis of spectral information available from the fingerprint region exhibited an excellent image contrast confirming standard histo- pathological examinations. The second approach included a systematic search for spectral windows which were supposed to contain the relevant information, necessary for spectra classification and identification. Data from these spectral windows were analyzed by an ANN and output data were utilized for image construction. In contrast to the PCA approach, the image contrast was lower although the main morphological structures were exactly classified. From the spectroscopic point of view, the spectral feature selection method delivered useful information which could be discussed in terms of structural alternations upon carcinogenesis.

Citation Download Citation

Peter Lasch, Wolfgang Waesche, W. J. McCarthy, Gerhard J. Mueller, and Dieter Naumann "Imaging of human colon carcinoma thin sections by FT-IR microspectrometry", Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); https://doi.org/10.1117/12.306083

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