Fluorescence-lifetime technologies for high-throughput screening

Todd E. French; John C. Owicki; Douglas N. Modlin; Sudhir S. Deshpande; I. Mineyev; Kimberly Crawford; Will Burton

doi:10.1117/12.307330

10 April 1998 Fluorescence-lifetime technologies for high-throughput screening

Todd E. French, John C. Owicki, Douglas N. Modlin, Sudhir S. Deshpande, I. Mineyev, Kimberly Crawford, Will Burton

Proceedings Volume 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery; (1998) https://doi.org/10.1117/12.307330
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

Fluorescence lifetime, the mean interval between absorption and emission, is as fundamental a characteristic of fluorescence as excitation and emission wavelengths and quantum yield. Yet, with the exception of time-resolved fluorescence assays utilizing lanthanide chelates, the analytical possibilities of methods based on fluorescence lifetime are virtually unexploited outside the academic research laboratory. We discuss the potential use of fluorescence-lifetime technologies in high-throughput screening from the standpoint of assay reagents and instrumentation. Among these applications are fluorescence- polarization assays based on long-lifetime probes and fluorescence-intensity assays using lifetime-resolved detection to reject background. We find that fluorescence- lifetime technologies offer significant practical advantages over existing methods.

Citation Download Citation

Todd E. French, John C. Owicki, Douglas N. Modlin, Sudhir S. Deshpande, I. Mineyev, Kimberly Crawford, and Will Burton "Fluorescence-lifetime technologies for high-throughput screening", Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); https://doi.org/10.1117/12.307330

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