Focused ion beams and life science applications: cell tomography and biomachining at ultrahigh resolution

Monica Ballerini; Marziale Milani; Michele Costato; I. C. Edmond Turcu; Franco Squadrini

doi:10.1117/12.307097

29 April 1998 Focused ion beams and life science applications: cell tomography and biomachining at ultrahigh resolution

Monica Ballerini, Marziale Milani, Michele Costato, I. C. Edmond Turcu, Franco Squadrini

Proceedings Volume 3260, Optical Investigations of Cells In Vitro and In Vivo; (1998) https://doi.org/10.1117/12.307097
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

A new technique of Focused Ion Beam (FIB) microscopy- nanomachining is proposed for life sciences. Its performances are compared with those of currently available ultramicroscopy apparatuses. Ultra-high resolution tridimensional tomography can be performed on whole cells without preparation. This can be achieved by sequentially etching layers of material and subsequently viewing the result of the operation under a different perspective. Very fast imaging times (minutes) allow quasi real time microscopy. The complementary technique of nano-biology can be performed on the same apparatus. The use of the ion beam allows to imaging both the surface and the inner part of the sample along any desired plane that can be chosen while the observation is on.

Citation Download Citation

Monica Ballerini, Marziale Milani, Michele Costato, I. C. Edmond Turcu, and Franco Squadrini "Focused ion beams and life science applications: cell tomography and biomachining at ultrahigh resolution", Proc. SPIE 3260, Optical Investigations of Cells In Vitro and In Vivo, (29 April 1998); https://doi.org/10.1117/12.307097

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available