Neuro3D: an interactive 3D reconstruction system of serial sections using automatic registration

Gregory Randall; Alicia Fernandez; Omar Trujillo-Cenoz; Gustavo Apelbaum; Marcelo Bertalmio; Luis Alberto Vazquez Rueda; Francisco Malmierca; Pablo Morelli

doi:10.1117/12.310542

9 June 1998 Neuro3D: an interactive 3D reconstruction system of serial sections using automatic registration

Gregory Randall, Alicia Fernandez, Omar Trujillo-Cenoz, Gustavo Apelbaum, Marcelo Bertalmio, Luis Alberto Vazquez Rueda, Francisco Malmierca, Pablo Morelli

Author Affiliations +

Proceedings Volume 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V; (1998) https://doi.org/10.1117/12.310542
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

3D detailed visualization of neurons represents a source of valuable information when trying to understand integrative phenomena at cellular and circuital levels. We describe here a low cost 3D reconstruction system developed by an interdisciplinary team of engineers and neurobiologists. The system allows 3D reconstruction of ultra thin sections observed through a transmission electron microscope. We have developed and tested a completely automatic registration method which combines local histogram equalization with correlation in multi-resolution. This method gives good experimental results in real images. It is not time consuming and it does not need dedicated hardware. The different parts of the system are briefly described: image acquisition, non-uniform illumination compensation, manual segmentation, automatic registration, 3D visualization and graphic user interface. Preliminary experimental results are also included here.

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Gregory Randall, Alicia Fernandez, Omar Trujillo-Cenoz, Gustavo Apelbaum, Marcelo Bertalmio, Luis Alberto Vazquez Rueda, Francisco Malmierca, and Pablo Morelli "Neuro3D: an interactive 3D reconstruction system of serial sections using automatic registration", Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); https://doi.org/10.1117/12.310542

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