3D microscopy with phase-shifting digital holography

Tong Zhang; Ichirou Yamaguchi

doi:10.1117/12.316445

6 July 1998 3D microscopy with phase-shifting digital holography

Tong Zhang, Ichirou Yamaguchi

Proceedings Volume 3479, Laser Interferometry IX: Applications; (1998) https://doi.org/10.1117/12.316445
Event: SPIE's International Symposium on Optical Science, Engineering, and Instrumentation, 1998, San Diego, CA, United States

Abstract

In this paper, we applied phase-shifting digital holography to microscopy. The digital holograms are recorded by a video camera from the superposition of a plane reference beam and the object beam passed through a microscopic objective. The distribution of complex amplitude of the object wave is derived by using the phase-shifting algorithm and then Fresnel transformed in a computer to reconstruct images at arbitrary planes. After relationships between object position and position of focused image and magnification are discussed, those are applied to determine the sizes and positions of small particles by image processing technique.

Citation Download Citation

Tong Zhang and Ichirou Yamaguchi "3D microscopy with phase-shifting digital holography", Proc. SPIE 3479, Laser Interferometry IX: Applications, (6 July 1998); https://doi.org/10.1117/12.316445

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