Determination of the optical properties of a human brain tumor using a new microspectrophotometric technique

Stefan Willmann; Albert Terenji; Ilya V. Yaroslavsky; Thomas Kahn M.D.; Peter Hering; Hans-Joachim Schwarzmaier M.D.

doi:10.1117/12.347503

30 April 1999 Determination of the optical properties of a human brain tumor using a new microspectrophotometric technique

Stefan Willmann, Albert Terenji, Ilya V. Yaroslavsky, Thomas Kahn M.D., Peter Hering, Hans-Joachim Schwarzmaier M.D.

Author Affiliations +

Proceedings Volume 3598, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications III; (1999) https://doi.org/10.1117/12.347503
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

We have developed a new technique to determine the optical properties of turbid media on a spatial scale of several 100 micrometers . This technique is based on transmission measurements into three different solid angles using a microspectrophotometer. An inverse Monte-Carlo simulation was employed to extract the optical properties from the sample under investigation. In this study, we investigated the optical properties of human brain tumor tissue. The transmission measurements were performed in the wavelength range from 400 nm to 800 nm for one spot on the sample. In addition, the histological inhomogeneity of the brain tumor was evaluated by probing the optical properties of different spots on the sample at a fixed wavelength. The resulting scattering coefficients varied between 6.0 and 7.9 mm^-1, the absorption coefficients between 0.50 and 0.92 mm^-1, and the anisotropy factors between 0.867 and 0.895. These result suggest that this microspectrophotometric technique is suitable to determine the optical properties of an inhomogeneous turbid media as for example a human brain tumor sample with a spatial resolution unattainable by conventional spectrophotometric techniques.

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Stefan Willmann, Albert Terenji, Ilya V. Yaroslavsky, Thomas Kahn M.D., Peter Hering, and Hans-Joachim Schwarzmaier M.D. "Determination of the optical properties of a human brain tumor using a new microspectrophotometric technique", Proc. SPIE 3598, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications III, (30 April 1999); https://doi.org/10.1117/12.347503

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