Native fluorescence characterization of human liver abnormalities

Singaravelu Ganesan; S. Madhuri; Prakasa Rao Aruna; S. Suchitra; T. G. Srinivasan

doi:10.1117/12.348380

17 May 1999 Native fluorescence characterization of human liver abnormalities

Singaravelu Ganesan, S. Madhuri, Prakasa Rao Aruna, S. Suchitra, T. G. Srinivasan

Proceedings Volume 3599, Optical Diagnostics of Biological Fluids IV; (1999) https://doi.org/10.1117/12.348380
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Fluorescence spectroscopy of intrinsic biomolecules has been extensively used in biology and medicine for the past several decades. In the present study, we report the native fluorescence characteristics of blood plasma from normal human subjects and patients with different liver abnormalities such as hepatitis, leptospirosis, jaundice, cirrhosis and liver cell failure. Native fluorescence spectra of blood plasma -- acetone extract were measured at 405 nm excitation. The average spectrum of normal blood plasma has a prominent emission peak around 464 nm whereas in the case of liver diseased subjects, the primary peak is red shifted with respect to normal. In addition, liver diseased cases show distinct secondary emission peak around 615 nm, which may be attributed to the presence of endogenous porphyrins. The red shift of the prominent emission peak with respect to normal is found to be maximum for hepatitis and minimum for cirrhosis whereas the secondary emission peak around 615 nm was found to be more prominent in the case of cirrhosis than the rest. The ratio parameter I₄₆₅/I₆₁₅ is found to be statistically significant (p less than 0.001) in discriminating liver abnormalities from normal.

Citation Download Citation

Singaravelu Ganesan, S. Madhuri, Prakasa Rao Aruna, S. Suchitra, and T. G. Srinivasan "Native fluorescence characterization of human liver abnormalities", Proc. SPIE 3599, Optical Diagnostics of Biological Fluids IV, (17 May 1999); https://doi.org/10.1117/12.348380

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