Three-dimensional superresolution imaging of single molecules by spectrally selective confocal microscopy

G. J. Brakenhoff; A. M. Van Oijen; J. Kohler; Michiel Muller; J. Schmidt

doi:10.1117/12.347588

6 May 1999 Three-dimensional superresolution imaging of single molecules by spectrally selective confocal microscopy

G. J. Brakenhoff, A. M. Van Oijen, J. Kohler, Michiel Muller, J. Schmidt

Proceedings Volume 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI; (1999) https://doi.org/10.1117/12.347588
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be realized. The feasibility of the idea is demonstrated with NA equals 0.55 optics on a model system of pentacene molecules in p-terphenal host matrix.

Citation Download Citation

G. J. Brakenhoff, A. M. Van Oijen, J. Kohler, Michiel Muller, and J. Schmidt "Three-dimensional superresolution imaging of single molecules by spectrally selective confocal microscopy", Proc. SPIE 3605, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, (6 May 1999); https://doi.org/10.1117/12.347588

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