QLF monitoring of therapies for early secondary caries arrestment and remineralization

Margherita Fontana; Carlos Gonzalez-Cabezas; George K. Stookey

doi:10.1117/12.380845

24 March 2000 QLF monitoring of therapies for early secondary caries arrestment and remineralization

Margherita Fontana, Carlos Gonzalez-Cabezas, George K. Stookey

Proceedings Volume 3910, Lasers in Dentistry VI; (2000) https://doi.org/10.1117/12.380845
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Secondary caries (SC) is the most common reason for restoration failure. The purpose of this study was to evaluate the Quantitative Light-Induced Fluorescence (QLF) method for monitoring therapies to inhibit SC progression. Forty-eight human teeth with resin restorations were demineralized for 4 days in a microbial caries model. Half of each specimen was then covered with an acid-resistant varnish to maintain the baseline lesion, and treated (group 1: non-treated control; group 2: chlorhexidine varnish for 24 h; group 3: fluoride varnish for 24 h; group 4: APF topical fluoride gel for 4 min), prior to being demineralized for 4 more days. Specimens were analyzed by QLF, sectioned, stained with Rhodamine B, and analyzed with a confocal microscope (CLSM) for lesion depth. The QLF results indicated that the control group was significantly (p less than 0.05) different (i.e., lesions progressed) from groups treated with fluoride (groups 3 and 4; lesions remineralized). All other group comparisons were not significantly different. Results obtained from CLSM analysis were similar to the ones obtained with QLF, except that lesions in group 2 were significantly deeper than the ones in the fluoride groups. Results suggest that the QLF method has a clear potential for monitoring remineralizing therapies for SC.

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Margherita Fontana, Carlos Gonzalez-Cabezas, and George K. Stookey "QLF monitoring of therapies for early secondary caries arrestment and remineralization", Proc. SPIE 3910, Lasers in Dentistry VI, (24 March 2000); https://doi.org/10.1117/12.380845

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KEYWORDS

Dental caries

Teeth

Composites

Luminescence

Therapeutics

Confocal microscopy

In vitro testing

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