Spatially resolved diffuse reflectance with laser Doppler imaging for the simultaneous in-vivo measurement of tissue perfusion and metabolic state

Kevin R. Forrester; Roxane Shymkiw; John Tulip; Craig Sutherland; David Hart; Robert C. Bray

doi:10.1117/12.388059

13 June 2000 Spatially resolved diffuse reflectance with laser Doppler imaging for the simultaneous in-vivo measurement of tissue perfusion and metabolic state

Kevin R. Forrester, Roxane Shymkiw, John Tulip, Craig Sutherland, David Hart, Robert C. Bray

Author Affiliations +

Proceedings Volume 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical; (2000) https://doi.org/10.1117/12.388059
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Laser Doppler Imaging (LDI) has become an established technique for the two dimensional measurement of tissue perfusion but the uncertainty of photon penetration depth leads to ambiguous interpretation of what fraction of the tissue microcirculation is being sampled. This study investigates a diffuse reflectance technique for measuring tissue optical properties during LDI perfusion measurement for the simultaneous determination of photon penetration depth and tissue metabolic state. LDI and diffuse reflectance spectroscopy measurements were made on surgically exposed ligaments in pregnant and non-pregnant rabbits. Photon penetration depths are reported. It was observed that anisotropic scattering occurs due to the ordered alignment of collagen fibers within ligament. Tissue perfusion in the ligaments of pregnant animals was significantly lower than in non-pregnant animals. Tissue hemoglobin concentration and oxygenation, and percent vascularization are also reported showing no statistical difference between the ligaments in pregnant and non-pregnant rabbits. A significant difference was observed in the photon scattering coefficient between the pregnant and non-pregnant groups suggesting a change in fibril spacing and/or orientation, most likely caused by an increased laxity in the ligaments of the pregnant animals. These investigations compare well with previous biochemical and biomechanical information obtained on ligaments.

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Kevin R. Forrester, Roxane Shymkiw, John Tulip, Craig Sutherland, David Hart, and Robert C. Bray "Spatially resolved diffuse reflectance with laser Doppler imaging for the simultaneous in-vivo measurement of tissue perfusion and metabolic state", Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); https://doi.org/10.1117/12.388059

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