Tomographic microscope with superimposition of fluorescence and interference contrast

Lidia I. Poplevina; Theodor V. Bulygin; Gennady G. Levin; Gennady N. Vishnyakov; Elena V. Pecherskih

doi:10.1117/12.384179

2 May 2000 Tomographic microscope with superimposition of fluorescence and interference contrast

Lidia I. Poplevina, Theodor V. Bulygin, Gennady G. Levin, Gennady N. Vishnyakov, Elena V. Pecherskih

Proceedings Volume 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII; (2000) https://doi.org/10.1117/12.384179
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Fluorescence microscopy is a powerful instrument for the study of cells and other biological objects. One of the actual tasks of the modern microscopy is the study of drugs transportation in cells and it's affect on the different cell organelles. The authors have proposed and tested the new type of the microscope with superimposed interference and fluorescence images of the living cell. The optical setup of the fluorescence interference microscope is based on confocal Linnik's type interference microscope. The fluorescence image gives the information about the distribution of drugs in different parts of the cell. The interference image gives the information about the distribution of dry mass or protein in the different parts of the cell.

Citation Download Citation

Lidia I. Poplevina, Theodor V. Bulygin, Gennady G. Levin, Gennady N. Vishnyakov, and Elena V. Pecherskih "Tomographic microscope with superimposition of fluorescence and interference contrast", Proc. SPIE 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII, (2 May 2000); https://doi.org/10.1117/12.384179

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