Orthogonal polarization spectral (OPS) imaging: a new technique for the visualization and study of microcirculation

A. G. Harris; I. Sinitsina; S. Pahernik; S. Langer; E. von Dobschuetz; P. Biberthaler; E. Uhl; O. Genzel; K. Messmer

doi:10.1117/12.431552

4 May 2001 Orthogonal polarization spectral (OPS) imaging: a new technique for the visualization and study of microcirculation

A. G. Harris, I. Sinitsina, S. Pahernik, S. Langer, E. von Dobschuetz, P. Biberthaler, E. Uhl, O. Genzel, K. Messmer

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Proceedings Volume 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II; (2001) https://doi.org/10.1117/12.431552
Event: Saratov Fall Meeting 2000, 2001, Saratov, Russian Federation

Abstract

OPS-imaging is a novel technique which can be used to obtain images of the microcirculation using reflected light. High contrast transillumination quality images can be collected not only from thin tissues, but from the surface of solid organs as well. In OPS-imaging the tissue is illuminated with light that has been linearly polarized in one plane. The light is then both scattered and reflected by the tissue. In front of the camera there is a second polarizer which is oriented in a plane precisely orthogonal to that of the illuminating light. This means that light which is directly reflected by the tissue, which maintains its polarization, is rejected by the polarizer in front of the camera. The only light which enters the camera and forms the image is light which has become depolarized, which typically requires at least 10 scattering events. Thus, the light which forms the image comes from deep (0.5 mm) within the tissue and effectively back-illuminates the absorbing material in the foreground. When the light has a wavelength within the hemoglobin absorption spectrum (548 nm), the scattered light is absorbed by the hemoglobin in the red cells, making it possible to visualize the blood vessels as in transillumination intravital microscopy. Thus, images of the microcirculation of solid organs can be obtained without the use of fluorescent dyes. OPS-imaging has been incorporated into a small, hand held device which is easily transportable (CYTOSCAN). Because of these two advantages, it is possible to not only use OPS-imaging in the laboratory, but also in the clinic on patients.

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A. G. Harris, I. Sinitsina, S. Pahernik, S. Langer, E. von Dobschuetz, P. Biberthaler, E. Uhl, O. Genzel, and K. Messmer "Orthogonal polarization spectral (OPS) imaging: a new technique for the visualization and study of microcirculation", Proc. SPIE 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II, (4 May 2001); https://doi.org/10.1117/12.431552

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KEYWORDS

Tissues

Microscopy

Capillaries

Luminescence

Cameras

Blood

Light scattering

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