Morphometric comparison of the acute rabbit and pig corneal response to 1540-nm laser light following ex-vivo exposure

Thomas E. Eurell; William P. Roach; Thomas E. Johnson

doi:10.1117/12.426718

17 May 2001 Morphometric comparison of the acute rabbit and pig corneal response to 1540-nm laser light following ex-vivo exposure

Thomas E. Eurell, William P. Roach, Thomas E. Johnson

Author Affiliations +

Proceedings Volume 4246, Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment; (2001) https://doi.org/10.1117/12.426718
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Single pulse, 1540 nm laser light with a pulse width of 1 microsecond altered the morphologic appearance of explant rabbit and pig corneas following ex vivo exposure. Using digital images of the post-exposure corneas projected onto a measuring grid, we could accurately locate the relative position of the circular laser lesion in the embedded tissue. This allowed us to section through the lesion with micrometer precision and accurately resolve the inside edge, middle and outside edge of the laser lesion. All tissue sections used for morphometric analysis were taken through the middle of the lesion. Several features of the response to laser exposure may reflect species-specific tissue differences. The rabbit corneal epithelium showed a homogeneous coagulative necrosis with a distinct demarcation between necrotic and normal epithelium. The pig epithelium also showed a distinct demarcation between necrotic and normal epithelium, however, there were several remarkable differences in the tissue response between the two species including coagulative necrosis pattern and nuclear morphology. These changes suggested a different and less severe response of the pig epithelium to the laser light when compared to the rabbit epithelium.

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Thomas E. Eurell, William P. Roach, and Thomas E. Johnson "Morphometric comparison of the acute rabbit and pig corneal response to 1540-nm laser light following ex-vivo exposure", Proc. SPIE 4246, Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment, (17 May 2001); https://doi.org/10.1117/12.426718

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KEYWORDS

Cornea

Laser vision correction

Laser tissue interaction

Pulsed laser operation

Tissues

Infrared lasers

Surgery

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