Electrical and optical methods for the manipulation and analyses of single cells

Daniel T. Chiu; Maximilian Davidson; Anette Stroemberg; Frida Ryttsen; Owe Orwar

doi:10.1117/12.426752

22 May 2001 Electrical and optical methods for the manipulation and analyses of single cells

Daniel T. Chiu, Maximilian Davidson, Anette Stroemberg, Frida Ryttsen, Owe Orwar

Proceedings Volume 4255, Clinical Diagnostic Systems; (2001) https://doi.org/10.1117/12.426752
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

This paper describes the use of focused electric fields and focused optical fields for the high-resolution manipulation of single cells. A focused electric field, obtained with the use of ultramicroelectrodes (tip diameter approximately 5 μm), is used to electroporate and electrofuse individual cells selectively and with high spatial resolution. A focused optical field, in the form of an optical tweezer, is used to isolate single organelles from a cell as well as to position liposomes incorporated with receptors and transporters along the cell for the high-resolution sampling and probing of the cellular microenvironment.

Citation Download Citation

Daniel T. Chiu, Maximilian Davidson, Anette Stroemberg, Frida Ryttsen, and Owe Orwar "Electrical and optical methods for the manipulation and analyses of single cells", Proc. SPIE 4255, Clinical Diagnostic Systems, (22 May 2001); https://doi.org/10.1117/12.426752

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