Observation of real-time interactions of Bcl-2 family members during apoptosis

Brian Herman; Victoria Frohlich; Ming Qiu; Akiyuki Takahashi

doi:10.1117/12.424549

24 April 2001 Observation of real-time interactions of Bcl-2 family members during apoptosis

Brian Herman, Victoria Frohlich, Ming Qiu, Akiyuki Takahashi

Proceedings Volume 4262, Multiphoton Microscopy in the Biomedical Sciences; (2001) https://doi.org/10.1117/12.424549
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Apoptosis is a physiological process of cell death resulting from an intricate cascade of sequential protein-protein interactions. Using donor and acceptor mutant GFP fusion constructs, we have monitored the interaction between specific pro- and anti-apoptotic members of the Bcl-2 family with each other as well as proteins located in the outer mitochondrial membrane, as current hypotheses regarding apoptosis suggest that interaction of Bcl-2 family members with each other, or with other mitochondrial membrane proteins, regulates apoptosis. Our data indicate that specific interactions between pro- and anti-apoptotic Bcl-2 family members do occur in situ in the mitochondrial membrane, are altered during apoptosis and regulate cellular sensitivity to apoptosis. These findings are the first to demonstrate real time protein-protein interactions in situ at the level of individual mitochondria.

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Brian Herman, Victoria Frohlich, Ming Qiu, and Akiyuki Takahashi "Observation of real-time interactions of Bcl-2 family members during apoptosis", Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); https://doi.org/10.1117/12.424549

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KEYWORDS

Cell death

Fluorescence resonance energy transfer

Proteins

Data modeling

Luminescence

Image filtering

Molecular interactions

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