Effect of low-power HeNe laser irradiation in vitro on intracellular calcium concentration and cellular activity in mouse macrophages

Chaoyang Xu; Shici Tan; Da Xing

doi:10.1117/12.462543

12 April 2002 Effect of low-power HeNe laser irradiation in vitro on intracellular calcium concentration and cellular activity in mouse macrophages

Chaoyang Xu, Shici Tan, Da Xing

Author Affiliations +

Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462543
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China

Abstract

Low level He-Ne laser irradiation (e.g., intravascular laser irradiation on blood, ILIB) has been widely used to treat some illness clinically. One of the action processing is by improving immunocompetence of the body, but the mechanism is still not clear. In this paper, we used mouse macrophages as study object for real-time observation of the concentration change of intracellular free calcium (abb. as [Ca²⁺]_i) of macrophages and the distribution of the Ca²⁺ using LSCM (laser scanning confocal microscope) after irradiation with different dose low level He-Ne laser (the actual irradiation power (abb. as P) is 0.16 mw and the facular diameter (abb. as D) is 0.5 cm. We found interesting phenomena through the image of [Ca²⁺]_i of macrophages. The distribution of Ca²⁺ in macrophages has no obvious change when the time of irradiation (abb. as T_i) is 10 min. The [Ca²⁺]_i shows obvious increase and a concentrate phenomena in Ca²⁺ distribution appears when T_i reach 20 min. The change of the distribution of Ca²⁺ become more distinct: [Ca²⁺]_i increase more visibly and the distribution of Ca²⁺ shows a spacial grads when T_i reach 30 min.

Citation Download Citation

Chaoyang Xu, Shici Tan, and Da Xing "Effect of low-power HeNe laser irradiation in vitro on intracellular calcium concentration and cellular activity in mouse macrophages", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462543

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